منابع مشابه
Effect of Different Doses of Staurosporine For Neuronal Differentiation
Purpose: The present study was designed to investigate the effect of different doses of Staurosporine on differentiation of PC-12 cell line into differentiated neuronal cells. Materials and Methods: PC-12 cell line as a useful model system for neurobiological studies was maintained in RPMI 1640 supplemented with 10% FBS. Staurosporine were added to culture medium in different concentrations (1...
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Fully understanding the phenotype of neurons in vivo involves examining their morphology, immunocytochemically analyzing their protein expression, examining their afferent and efferent integration into neuronal microcircuitry, and functionally examining their activity. This task is significantly more difficult when you are attempting to determine whether multipotent precursor cells, often refer...
متن کاملNrf2 promotes neuronal cell differentiation.
The transcription factor Nrf2 has emerged as a master regulator of the endogenous antioxidant response, which is critical in defending cells against environmental insults and in maintaining intracellular redox balance. However, whether Nrf2 has any role in neuronal cell differentiation is largely unknown. In this report, we have examined the effects of Nrf2 on cell differentiation using a neuro...
متن کاملInduction of Human Embryonic Stem Cells into neuronal differentiation by increasing cyclic Adenosine Mono Phosphate
Introduction: To evaluate the cAMP -mediated IBMX (3-IsoButyle -1-Methyl Xanthin) and db-cAMP (dibutyryl cAMP) effects on differentiation of human Embryonic Stem Cells (hESCs) into nerve cells were the objectives of this study. Methods: We have used Royan H1 hESC- derived embryoid bodies with four treatment groups: six days treatment with IBMX (5×10 -4M) and db-cAMP (10 -9M) (referred to as...
متن کاملNeuronal differentiation of embryonic stem cells.
Neuronal differentiation from totipotent precursors in vitro, is thought to require two signals: first a biophysical state (cellular aggregation) followed by a biochemical signal (retinoic acid treatment). In investigating the properties of retinoic acid-differentiated embryonic stem cell lines. However, we noted that retinoic acid treatment without prior aggregation, is sufficient to induce ex...
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ژورنال
عنوان ژورنال: Cell
سال: 2004
ISSN: 0092-8674
DOI: 10.1016/j.cell.2004.09.024